ActiveMax® Human Cytokines

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ActiveMax® Human Cytokines is our flagship product line featuring a complete collection of commonly used human cytokines and growth factors. Unlike most of other commercially available cytokine products, ActiveMax® cytokines are expressed exclusively in human cells under strict animalfree and xeno-free conditions. Therefore, they are uniquely suitable for human cell culture and assay development.

Here are some of the reasons why you should choose ActiveMax® cytokines for your studies:

 

Authentic Human Protein

Gel filtration analysis of HEK293-expressed ActiveMax®

The function of a protein heavily depends on its post translational processing, such as glycosylation, folding, and subunit assembly. Human recombinant proteins expressed from bacteria, insect, or non-human mammalian cells often fall short in fully recapitulating the post-translational modifications of their native counterparts, largely due to lack of human-specific Proliferation of TF-1 cells assayed 5 days after treatment with HEK293-expressed ActiveMax®mechanisms in the hosts. Consequently, these proteins often demonstrate compromised biological properties.

 

 

On the contrary, the production of ActiveMax® cytokines relies entirely on the human cellular machinery provided by the HEK293 cells. As a result, our cytokines retain their authentic post-translational processing (Fig. 1), which translates into high bioactivity and stability (Fig. 2). In addition, the HEKMax® system confers very high level of target expression, which allows us to purify proteins without the use of artificially introduced tags. This true tag-free production brings the benefit of fully maintaining the proteins’ native conformation and biological properties, including ligand-receptor binding affinity, which may be affected by subtle charge/size changes caused by the addition of tags.

 

ActiveMax Table

 

Xeno-Free and Endotoxin-Free

Endotoxin and other non–human components can provoke immune response in human systems. These contaminations are significant complicating factors for human cell culture experiments, in particular those relevant to human cell therapy or other clinical applications. Our ActiveMax® cytokines are exclusively produced in a serum-free, animal component-free, and chemically defined cell culture system. These cytokines are absolutely xeno-free, and have an endotoxin level of less than 1.0 EU per μg.

 

Bulk Production

Our HEKMax® technology platform employs a high density cell culture system, specially designed expression vectors, and proprietary HEK293 cell lines with a low background and an optimal metabolic profile. The seamless integration of these elements allows us to easily scale up our production to meet your demand for bulk amount of cytokines. With this refined expression system in place , you can enjoy the benefits of an industrial production process with a highly affordable price.

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