DNeasy mericon Food Kit (69514)

 DNeasy mericon Food Kit (69514)

DNeasy mericon Food Kit


During production, food is subjected to heat, irradiation, high pressure, and changes in pH which lead to DNA degradation and fragmentation. The optimized chemistry of the DNeasy mericon Food Kit was developed to recover short DNA fragments (down to 100 bp) ensuring that even highly fragmented DNA is efficiently isolated and subsequently amplified in PCR reactions. Thanks to these features, this kit offers a universally applicable extraction method that generates optimal and reliable results with even highly processed, fatty, or acidic foods, or foods with high or low DNA contents or high inhibitor contents (see the figures “High yields of DNA with minimal inhibitor carryover”).
Principle

DNeasy mericon Food Kit

This kit uses an improved cetyltrimethylammonium bromide (CTAB) extraction of total cellular nucleic acids. The nonionic detergent CTAB is widely used for efficient extraction of total cellular nucleic acids from a wide range of tissue types. Depending on the salt conditions, CTAB may complex with cellular nucleic acids (low-salt conditions) or complex with cellular inhibitors, such as polysaccharides, proteins, and plant metabolites (high-salt conditions; as found in the Food Lysis Buffer).

With fewer workflow steps than conventional CTAB protocols, up to 30 samples can be processed in 2.5 hours with the DNeasy mericon Food Kit (see the figure “Efficient and rapid DNA extraction”).
The optimized protocol uses CTAB in combination with Proteinase K to first digest compact tissue and then to subsequently precipitate proteins with simultaneous precipitation of other cellular and food-derived inhibitors.

Inhibitors are precipitated by centrifugation, while the extracted DNA remains in solution. In the subsequent chloroform extraction, any remaining CTAB-protein, CTAB-debris, or CTAB-polysaccharide complex not precipitated is removed together with other lipophilic inhibitors by extraction into the organic chloroform phase. Only the aqueous phase containing the DNA and significantly depleted inhibitors is processed further. This phase is mixed with binding buffer (to adjust binding conditions) and applied to the QIAquick Spin Columns. The resulting DNA is immediately ready for use in a downstreammericon real-time PCR assay.

 

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