Feline Infectious Peritonitis Virus RT-PCR Detection Kit

Feline Infectious Peritonitis Virus RT-PCR Detection Kit

Isolation and detection of Feline Infectious Peritonitis Virus (FIPV) from blood samples using end-point one-step RT-PCR.

Feline infectious peritonitis (FIP) is a viral disease of cats caused by the Feline Infectious Peritonitis Virus (FIPV), which is a strain of feline coronavirus. Most strains of feline coronavirus are avirulent, which means that they do not cause disease, and are referred to as feline enteric coronavirus. Cats infected with a feline coronavirus generally do not show any symptoms during the initial viral infection. Feline coronaviruses can be found in large quantities in the saliva and feces of cats during the acute infection and to a lesser extent in recovered or carrier cats, so it can be transmitted through cat-to-cat contact and exposure to feces.

In a small percent of infected cats (5 to 10 percent), the infection progresses into clinical FIP either by a mutation of the virus or by an aberration of the immune response. The virus is then referred to as feline infectious peritonitis virus (FIPV), and typically higher concentrations of the virus can then be detected in blood samples.
Cats that have been initially exposed to the feline coronavirus usually show no obvious symptoms. Once symptoms develop, often there is increasing severity over the course of several weeks, ending in death. Any cat that is a carrier of any coronavirus is at risk of developing FIP. Cats with weakened immune systems, kittens, cats already infected with feline leukemia virus and geriatric cats are especially at risk. There is no known cure or effective treatment for FIP at this time.
• Isolate High Quality, Inhibitor-Free RNA – Removal of highly concentrated salts, metabolic wastes and proteins provides high quality, concentrated viral RNA.
• Rapid Procedure – Isolate and detect Feline Infectious Peritonitis Virus in under 3 hours.
• Accuracy in Interpreting Results – Identify possible PCR inhibition and/or inadequate isolation with the provided RT-PCR Control Master Mix
• High Sensitivity and Specificity – The limit of detection of the FIPV 2X RT-PCR Master Mix is 100 copies per reaction.
• Purchase Primer Set and Controls Separately – The FIPV Primer set as well as the positive control and negative control can be purchased separately