Genomic DNA Purification Kit (K0512)

Genomic DNA Purification Kit (K0512)

Description

Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

• Efficient—2 to 10 µg of genomic DNA from 0.2 mL of blood
• Fast—procedure takes approximately 20 minutes
• Universal—purifies genomic DNA from various sources
• Flexible—easy to scale up and down
• Safe—does not involve phenol extraction

Applications

• Purification of high-quality genomic DNA from:
• Whole blood or serum
• Cell culture
• Plant tissues
• Mammalian tissues
• Epithelium samples
• Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol.

Specifications

Sample Type (General): Liquid Samples (e.g. Serum), Plant Samples, Tissue, Bacteria, Yeast, Cells, Blood
Downstream Application: PCR, Cloning, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Product Size: 100 preps