Gentra Puregene Tissue Kit REF. 158667

 Gentra Puregene Tissue Kit REF. 158667

For purification of archive-quality DNA from tissues

  • Archive-quality DNA for long-term storage
  • Reproducible DNA purification
  • A complete solution for sample-to-storage purification
  • Convenient, scalable purification procedure

The Gentra Puregene Tissue Kit enables purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.

 

Principle

Cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.

 

 Feature Specifications

 

ApplicationsPCR, restriction digest, Southern analysis, SNP analysisElution volume50-250 µl (varies)FormatScalableMain sample typeTissue samples, fixed and paraffin-embeded tissueProcessingManualPurification of total RNA, miRNA, poly A+ mRNA, DNA or proteinGenomic DNASample amount5-100 mgTechnologyModified salting-out precipitation methodTime per run or per prep25 – 60 minutes (+ cell lysis + DNA rehydration)YieldVaries

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