Maxima Probe qPCR Master Mix

Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Catalog number: K0261

Description

Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (see Reference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

Highlights

  • Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity—detects low copy number targets
  • Wide linear range—accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience—ready-to-use 2X master mix
  • Applications
  • Gene expression
  • siRNA validation
  • Genotyping
  • Pathogen detection

Includes

Maxima Probe qPCR Master Mix, ROX Solution provided (2X) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer. Supplied with nuclease-free water and a separate ROX vial.

Specifications

Detection Method: Primer-Probe Detection
Platform: Cepheid SmartCycler, BioRad iQ5, BioRad iCycler iQ, BioRad MyiQ, MJ Opticon, BioRad CFX96™, Roche LightCycler 480, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Corbett RotorGene, MJ Chromo4
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): dsDNA, DNA
Polymerase: Taq DNA Polymerase
Product Size: 200 reactions
Number of Reactions: 200 Reactions

Contents & storage

Contains:

2 x 1.25 mL Maxima Probe qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer; sufficient for 200 x 25 µL reactions.

50 µL ROX solution, 50 µM

2 x 1.25 mL Nuclease-Free Water

Store at -20°C.