Mericon DNA Bacteria Plus Kit (69534)

 Mericon DNA Bacteria Plus Kit (69534)

Mericon DNA Bacteria and DNA Bacteria Plus Kits

The thermal lysis protocol of the mericon DNA Bacteria Kit and the mechanical lysis protocol of the mericon DNA Bacteria Plus Kit are similarly efficient for the extraction of DNA from Gram-negative bacteria, while the mechanical lysis protocol is more effective for the extraction of DNA from Gram-positive bacteria (see the figure “Efficient bacterial DNA extraction”).

Principle

Mericon DNA Bacteria Kit and mericon DNA Bacteria Plus Kit

To date, the predominant method to test food for the presence of pathogens has been via culture preparation and/or ELISA-based assays. Molecular-based methods, specifically real-time PCR, offer an informative complement to these well-established methods.

The thermal lysis protocol of the mericon DNA Bacteria Kit is optimized for the majority of easily lysed Gram-negative bacteria (e.g., Salmonella spp., Camplylobacter spp., and Cronobacter spp.). The mechanical lysis protocol of the mericonDNA Bacteria Plus Kit creates the appropriate conditions for bacterial extraction from Gram-positive bacteria, such asListeria spp.

The carefully formulated chemistry included in the mericon DNA Bacteria Kit and the mericon DNA Bacteria Plus Kit maximizes lysis efficiency, stabilizes the extracted DNA, and removes inhibitors that may interfere with downstream applications.