mericon Salmonella spp Kit (24) (288305)

Mericon Salmonella spp Kit (24) (288305)

Performance

mericon Salmonella spp Kit
The mericon Salmonella spp Kit is for the specific detection of several Salmonella subspecies in enrichment cultures of food and animal feed samples. The assay can detect as few as 10 copies of Salmonella DNA in a reaction and it exhibits high specificity for pathogens of this genus. In an experiment using 2500 copies of the tested DNA, no cross-reactivity was observed with other pathogens (see the table “Results of mericon Salmonella spp Kit cross-reactivity study”).

Results of mericon Salmonella spp Kit cross-reactivity study
Pathogen Result Pathogen Result
Salmonella spp + Cronobacter sakazakii
Clostridium perfringens Bacillus cereus
Staphylococcus aureus Legionella erythra
Listeria monocytogenes Campylobacter jejuni
Yersinia enterocolitica Legionella pneumophila
Shigella flexneri VTEC stx1
Escherichia coli VTEC stx2

Principle

Detection of pathogens using real-time PCR is based on the amplification of a specific region of the relevant pathogen genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.

mericon PCR Assays include an internal control to control potential inhibiton. This aids in the straightforward interpretation of the results (see the table “Summary of possible PCR outcomes”).

The PCR primer set for each assay is highly specific and targets a unique and conserved region of the tested pathogen genome that has been bioinformatically and experimentally verified.

Summary of possible PCR outcomes
Internal control amplified Tested DNA amplified Meaning
+ + Sample is positive for the target DNA
+ Sample is negative for the target DNA
PCR failed due to inhibition