N580 RIBOZOL™ RNA EXTRACTION REAGENT

Grade:BIOTECHNOLOGY GRADE
Ref.N580-200
Overview
Isolate intact total RNA from even the most difficult of cells and tissue types
Extract total RNA in less than one hour
Achieve consistent results and excellent recovery of small RNAs
Compatible with a variety of applications including Northern analysis, dot blots, cloning,in vitro translation, poly (A+) selectiion and RT-PCR
Detailed Description
Ribozol™ RNA Extraction Reagent is a single phase phenol solution optimized for the isolation of total RNA from a variety of cell and tissue types. Homogenization or disruption directly in Ribozol™ RNA Extraction Reagent inhibits RNase activity to substantially minimize degradation of all classes of RNA. The simple and effective procedure for isolation in Ribozol™ RNA Extraction Reagent includes homogenization, phase separation, RNA precipitation, RNA wash and solubilization.

Abs.@ Lambda Max

1.50 – 2.70

DNase (P/F)

Electrophoresis (P/F) (P/F)

Lambda Max (nm)

530 – 534

Percent OD260/280 (%)

95

Percent Yield (%)

80

pH @25C

3.8 – 4.2

RNase (P/F)

Specific Gravity @20C

1.0824 – 1.1124

Water Content (KF) (%)

36 – 42