QIAcube

 QIAcube

Ref. 9001292

 

Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: 
For fully automated sample prep using QIAGEN spin-column kits

  • Automation of trusted QIAGEN spin-column kits

  • Elimination of manual processing steps

  • Purification of DNA, RNA, or proteins

  • More free time with affordable automated processing

  • Standardized results and increased productivity

The innovative QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into the laboratory workflow. No change of purification chemistry is required, assuring fast startup and immediate results. All steps in the purification procedure are fully automated — up to 12 samples can be processed per run.

 

Principle

 

The QIAcube enables continued use of well-established QIAGEN spin-column kits and eliminates the need for tedious manual steps. The innovative QIAcube controls integrated components including a centrifuge, heated shaker, pipetting system, and robotic gripper. This enables the QIAcube to fully automate more than 40 QIAGEN spin-column kits.

The QIAcube is preinstalled with a variety of protocols for purification of RNA, genomic DNA, plasmid DNA, viral nucleic acids, and proteins, plus DNA and RNA cleanup. All standard protocols in the expanding range can also be downloaded free of charge. In addition, customized protocols tailored to meet your specific application demands can also be requested.

 

Efficient purification of viral nucleic acids

 

The QIAcube together with the QIAamp MinElute Virus Spin Kit enables efficient purification of viral nucleic acids. To evaluate the risk of sample-to-sample carryover during and between runs, the QIAamp MinElute Virus Spin procedure was subjected to rigorous testing using an alternating checkerboard setup of negative and highly positive plasma samples (1 x 108 IU/ml of a typical DNA virus). All of the highly positive samples were detected. All negative samples, in the checkerboard runs and the all-negative runs were unresponsive (see table “No sample carryover detected”).

Negative and highly positive (1 x 108 IU/ml typical DNA virus) plasma samples arranged in an alternating sequence on the QIAcube were purified using the QIAamp MinElute Virus Spin Kit and analyzed by real-time RT-PCR. Mean CT value for positive samples was 19. Samples with CT > 45 were regarded as negative. Under these conditions sample carryover was not detected.