QIAquick PCR Purification Kit (250) Ref. 28106

 QIAquick PCR Purification Kit (250) Ref. 28106

For purification of up to 10 μg PCR products, 100 bp to 10 kb

  • Up to 95% recovery of ready-to-use DNA
  • Cleanup of DNA up to 10 kb in three easy steps
  • Gel loading dye for convenient sample analysis

The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube.

PERFORMANCE

The QIAquick PCR Purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples (see figure Complete primer removal after PCR). Using a microcentrifuge or vacuum manifold, DNA ranging from 100 bp to 10 kb is purified.

PRINCIPLE

QIAquick Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples (see figure Complete primer removal after PCR). Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.

APPLICATIONS

DNA fragments purified with the QIAquick system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.

 

SPECIFICATIONS

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