QuantiTect SYBR® Green PCR Kits Ref. 204143

 QuantiTect SYBR® Green PCR Kits Ref. 204143

Performance

 

QuantiTect SYBR Green PCR Kits allow specific quantification over a wide linear range (see figure Specific quantification over a wide linear range). HotStarTaq DNA Polymerase provides the most stringent hot start compared with other polymerases, further increasing the specificity of the reaction (see figure Highly specific amplification). The unique composition of PCR buffer and HotStarTaq DNA Polymerase enable quantification of even low-abundance transcripts — as few as 5 copies of a target can be accurately detected (see figure Highly sensitive quantification). When used in combination with the QuantiTect Reverse Transcription Kit and QuantiTect Primer Assays, the QuantiTect SYBR Green PCR Kit delivers sensitive and reliable results (see figure Specific and sensitive quantification).

The combination of the specially optimized UNG solution and the proven PCR master mix in the QuantiTect SYBR Green PCR +UNG Kit ensures effective elimination of carried-over PCR products together with reliable quantification of target sequences (see figure High specificity in real-time PCR).

 

Principle

 

QuantiTect SYBR Green PCR Kits contain an optimized, ready-to-use master mix for highly specific and sensitive real-time quantification cDNA targets using SYBR Green I. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target-specific labeled probes. A balanced combination of K+ and NH4+ ions in the PCR buffer promotes specific primer annealing and enables high PCR specificity and sensitivity (see figure Specific primer annealing). In addition, HotStarTaq DNA Polymerase provides a stringent hot start, preventing the formation of nonspecific products.

QuantiTect SYBR Green PCR master mix also contains dUTP, enabling pretreatment with uracil-N-glycosylase (UNG) prior to starting PCR, which ensures that any contaminating PCR products do not affect subsequent PCR reactions.

 

Components of 2x QuantiTect SYBR Green PCR Kit

 

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