virotype BVDV RT-PCR Kit

virotype BVDV RT-PCR Kit

For safe detection of RNA from bovine viral diarrhea virus

The virotype BVDV RT-PCR Kit allows the safe detection of RNA from bovine viral diarrhea virus genotypes 1 and 2 (BVDV-1 and BVDV-2) in samples from cattle. The kit contains all of the reagents for real-time RT-PCR. It can be used with pools of up to 50 individual samples of blood, plasma, or serum, 25 individual samples of ear tissue, and 100 individual samples of milk.

• Rapid and specific detection of RNA from bovine viral diarrhea virus
• Amplification control included in PCR mix
• RT-PCR run time of less than 2.5 hours
• Can be performed in one cycler run with other virotype RT-PCR assays
The kit contains a ready-to-use PCR mix and enzyme mix and the required positive and negative controls. With this kit, both reverse transcription and PCR are performed in one reaction tube, reducing the risk of contamination.
RNA is extracted from the sample material (blood, plasma, serum, ear tissue, or milk) using a suitable sample preparation kit. The virotype Tissue Lysis Reagent provides a fast, easy-to-use, and convenient method for RNA preparation from ear tissue samples. The extracted RNA is used for the RT-PCR, which is run on a thermal cycler.
Principle:

This is a real-time RT-PCR kit for the detection of RNA from bovine viral diarrhea virus. RT-PCR is based on the amplification of specific regions of the pathogen genome. It is a sensitive and specific tool for pathogen detection.
Real-time RT-PCR involves the reverse transcription of viral RNA and subsequent amplification of the cDNA by PCR. The amplified product is detected using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating product without the need to re-open the reaction tubes afterward.

1