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Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: 
For fully automated sample prep using QIAGEN spin-column kits

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Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: 
For fully automated sample prep using QIAGEN spin-column kits

  • Automation of trusted QIAGEN spin-column kits

  • Elimination of manual processing steps

  • Purification of DNA, RNA, or proteins

  • More free time with affordable automated processing

  • Standardized results and increased productivity

The innovative QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into the laboratory workflow. No change of purification chemistry is required, assuring fast startup and immediate results. All steps in the purification procedure are fully automated — up to 12 samples can be processed per run.

Performance


Highly pure plasmid DNA


The QIAcube together with the QIAprep Spin Miniprep Kit enables fully automated purification of up to 20 µg molecular biology grade plasmid DNA. The fully automated purification procedure — including lysis — provides yields of plasmid DNA comparable to the manual procedure (see figure DNA yields comparable to the manual procedure). Purified DNA is suitable for use in routine molecular biology applications, such as fluorescent sequencing, cloning, or transfection of robust cells (see figure Reliable sequencing results).

High-quality genomic DNA


Using the QIAamp DNA Blood Kit, the QIAcube enables purification of reproducible yields of highly pure genomic DNA (see figure Reproducible DNA yields). DNA purified using the QIAcube performs well in sensitive PCR even when large amounts of eluate are used, demonstrating the high purity of the DNA (see figure High PCR performance).

Efficient purification of viral nucleic acids


The QIAcube together with the QIAamp MinElute Virus Spin Kit enables efficient purification of viral nucleic acids. To evaluate the risk of sample-to-sample carryover during and between runs, the QIAamp MinElute Virus Spin procedure was subjected to rigorous testing using an alternating checkerboard setup of negative and highly positive plasma samples (1 x 108 IU/ml of a typical DNA virus). All of the highly positive samples were detected. All negative samples, in the checkerboard runs and the all-negative runs were unresponsive (see table “No sample carryover detected”).

Principle
The QIAcube enables continued use of well-established QIAGEN spin-column kits and eliminates the need for tedious manual steps. The innovative QIAcube controls integrated components including a centrifuge, heated shaker, pipetting system, and robotic gripper. This enables the QIAcube to fully automate more than 40 QIAGEN spin-column kits. 

The QIAcube is preinstalled with a variety of protocols for purification of RNA, genomic DNA, plasmid DNA, viral nucleic acids, and proteins, plus DNA and RNA cleanup. All standard protocols in the expanding range can also be downloaded free of charge. In addition, customized protocols tailored to meet your specific application demands can also be requested. 
 
Association for Laboratory Automation’s (ALA) New Product Award (NPA)

The QIAcube - QIAGEN’s revolutionary new platform for sample preparation - receives the Association for Laboratory Automation’s (ALA) New Product Award (NPA).

DNA yields comparable to the manual procedure.

pCMVβ plasmid DNA was purified from 3 ml overnight cultures of E. coli (DH5α) grown in LB medium using the QIAprep Spin Miniprep Kit automated on the QIAcube or by using the manual procedure. DNA yields were determined spectrophotometrically. Automated DNA purification on the QIAcube gave comparable yields to the manual procedure.

Reliable sequencing results.

pUC19 Plasmid DNA was purified from a 1.5 ml overnight culture of E. coli grown in LB medium using the QIAprep Spin Miniprep Kit on the QIAcube and sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit. Sequencing reactions were purified using the DyeEx 96 Kit and analyzed on an ABI PRISM 3700 DNA Analyzer.

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QIACUBE

QIACUBE

Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits: 
For fully automated sample prep using QIAGEN spin-column kits

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