QIAgility

9001903

Nuevo producto

  1. For rapid, high-precision automated PCR setup
  • Automated PCR setup in all formats
  • Convenient, easy-to-use software
  • Standardized results and increased productivity
  • Elimination of manual pipetting steps
  • Seamless integration with QIAGEN sample technologies

Más detalles

Más

The QIAgility is a compact benchtop instrument that enables rapid, high-precision PCR setup. The unmatched versatility of the QIAgility means that almost all tube and plate formats are supported, as well as Rotor-Discs for the Rotor-Gene Q. The QIAgility is operated via a computer with easy-to-use software. For PCR success at first attempt, use verified Q Protocols for PCR setup in combination with QIAGEN PCR kits and enzymes for end-point and real-time PCR.

Performance

The advanced design of the QIAgility delivers improved process safety, giving you peace of mind. An in-built sensor ensures that the instrument pauses when the hood is opened during a run, protecting users from moving parts. A UV light ensures effective worktable decontamination, and a HEPA filter provides positive clean air pressure throughout PCR setup. These safety features are complemented by intelligent safety warnings from the software, should an error occur.  

In addition to PCR setup, the flexibility of the QIAgility allows the user to program an unrivaled range of high-precision pipetting applications (see table Speed and versatility), which includes: 

  • Normalization of DNA and RNA concentration
  • Transfer of liquid samples from one tube format to another
  • Serial dilutions with variable dilution ratios
  • Restriction digest setup
  • Sample pooling
  • Selective pipetting from archived sample banks

Table: Speed and versatility

Speed and versatility

Principle

The QIAgility uses a single-channel pipet to automate PCR setup — up to 96 PCR reactions can be set up in approximately 30 minutes. Liquid-level sensing, facilitated by conductive filtered tips, enables high-precision, standardized pipetting (see table Outstanding precision). Automated PCR setup is rapid and reliable and eliminates manual pipetting steps that can be prone to human error. The QIAgility fully automates trusted QIAGEN PCR and RT-PCR, and real-time PCR and RT-PCR kits, and can set up multiple master mixes and can process multiple PCR setups in parallel.

Table: Outstanding precision

The advanced design of the QIAgility delivers improved process safety, giving you peace of mind. An in-built sensor ensures that the instrument pauses when the hood is opened during a run, protecting users from moving parts. An optional UV light ensures effective worktable decontamination and an optional HEPA filter provides positive clean air pressure throughout PCR setup. These safety features are complemented by intelligent safety warnings from the software, should an error occur.

Procedure

The instrument worktable is set up with a Master Mix Block, a Reagent Block, tips and plates/tubes . The QIAgility software simulates the instrument worktable, making it exceptionally easy to use by both automation novices and more experienced laboratory personnel. Simply select a program, set up the worktable, and walk away during setup. Samples are ready for immediate use in downstream applications.
 

Accurate automated reaction setup.

IL12RB1 was amplified from leukocyte RNA (1, 10, and 100 ng), using the Rotor-Gene Q and the QuantiFast Probe RT-PCR Kit. Replicates of 3 were set up manually and using the QIAgility. DNA yields were quantified by quantitative, real-time RT-PCR, where the Rotor-Gene Q was also used for analysis.

Eliminate manual pipetting steps.

Prp, HugL, and Cyst were amplified from human genomic DNA (10 ng). PCR reactions were set up using the TopTaq Master Mix Kit, in replicates of 2, using the QIAgility and manually. After cycling, reactions were analyzed by capillary electrophoresis, using the QIAxcel.

Easy-to-use software

The worktable setup is mirrored in the main software window.

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QIAgility

QIAgility

  1. For rapid, high-precision automated PCR setup
  • Automated PCR setup in all formats
  • Convenient, easy-to-use software
  • Standardized results and increased productivity
  • Elimination of manual pipetting steps
  • Seamless integration with QIAGEN sample technologies